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A growing number of researches have shown that non-coding RNAs have powerful and diverse biological effects. However, little is known about the mechanisms of non-coding RNA function. To understand these mechanisms, it is important to define the interactions between ncRNA and cellular proteins. The identification of these ribonucleoprotein complexes is a key step in understanding how non-coding RNAs function. In vitro RNA pulldown techniques can be used to identify protein chaperone interests that interact with RNA.
The RNA pulldown assay selectively extracts protein-RNA complexes from the sample. In general, RNA pulldown assays take advantage of high-affinity tags (such as biotin, etc.) The RNA probe can be biotinylated to complex with proteins in the cell lysate and then purified using agarose or magnetic beads. Alternatively, the protein can be labeled or the RNA-protein complex can be isolated using an antibody to the protein of interest. RNA is then detected by Northern blot or RT-PCR analysis, and protein is detected by Western blot or mass spectrometry.
IntegrateRNA provides you with optimized RNA pulldown services, including biotin labeling and antibody labeling, to suit your interaction research project.
IntegrateRNA offers a variety of in vivo and in vitro systems for RNA functional studies to help our customers optimize project research solutions to increase success rates and reduce cycle times. We guarantee that all services and products are subject to strict quality control. Based on professional, efficient and experienced services, IntegrateRNA has won the unanimous trust and support of customers. If you have any questions, please feel free to contact us.