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RNA-mediated interference (RNAi) is a simple and rapid method for silencing gene expression in a variety of organisms, revolutionizing the way researchers conduct gene expression. RNAi technology enables the analysis of deletions in gene function in mammalian cells and animal models and significantly improves the convenience, speed, and specificity of this procedure. It has been a major choice for researchers to conduct functional deficit studies.
Currently, there are three main RNAi tools to perform these functions: small interfering RNA (siRNA), short hairpin RNA (shRNA), and microRNA (miRNA).
RNAi is fundamentally a two-step process. The first step involves the main enzyme in the RNAi process, type III endoribonase called Dicer. This enzyme is involved in the ATP-dependent digestion of longer dsRNA into 21 to 23 bp siRNA. In the second step, siRNA is loaded into the effector complex RNA-induced silencing complex (RISC) that recognizes the corresponding mRNA and cleaves it at a specific site, eventually (being eliminated) leading to a loss of protein expression.
Unlike siRNA, miRNA-loaded RISC complexes scan for potential complementarity of cytoplasmic mRNA. Instead of destructive cleavage, miRNAs target the 3' untranslated region (UTR) regions of mRNA, which usually bind with imperfect complementarity, thereby preventing translation of the ribosome.
Figure 1: Beyond interference (Erika Check. 2007)
RNAi technology can be efficiently used for target verification operations, allowing us to directly observe the function-deficient effects of specific genes in mammalian systems, identify the disease genes and assessed their correlated function. RNAi technology is not only used in related studies of lead compounds or signaling pathways, but also being used as a treatment for a variety of diseases for potential therapeutic applications, including macular degeneration, hepatitis, AIDS, Huntington's disease and cancers.
IntegrateRNA is committed to building effective systems and procedures to meet the needs and schedules of customers working with RNAi. IntegrateRNA provides a series of RNAi services, including in vitro synthesis of interfering RNA, vector-based RNAi services, and RNAi viral packaging, to help you identify the most potent RNA sequences with powerful gene silencing capabilities to establish stable cell lines and observe the long-term knockdown effect of their target genes. Our RNAi-related services will help advance your project research and enhance your organization's ability to make informed decisions about important milestones.
Vector-Based miRNA Service
Based on the National Technology Platform and an excellent team of experts, we are committed to providing our customers with the most reliable and efficient research services to best match their research objectives.