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qPCR array technology, also known as gene function classification chip or PCR array, combines the high sensitivity and high specificity of real-time PCR technology and microarray technology to simultaneously detect the advantages of multiple genes. It is an analysis of signaling pathways or organisms. The tool of choice for learning functionally related gene expression status. qPCR Arrays save experimental researchers a number of cumbersome processes, such as pre-experimental, primer validation, and literature selection genes. It provides direct insight into the expression profiles of key genes in specific signaling pathways or diseases in different research topics.
They can be used in 96-well, 384-well and (hopefully fast) mRNA and microRNA expression profiles in the 1536-well format. The detection chemistry used in the qPCR array is SYBR Green I (or a similar DNA binding dye) or an optimized probe-based primer set (primarily a TaqMan probe or an LNA probe) (Table 1). They can screen entire expressed microRNAs or sub-panels, such as pathways or gene families associated with diseases. qPCR arrays can also be customized to include a set of genes tailored to your specific research interests. High-quality primer design and qPCR Master mix allow PCR array to simultaneously amplify 96 or 384 different gene-specific products under uniform cycling conditions.
Table 1: Characteristics of the two methods
Gene expression analysis by PCR arrays has the sensitivity of real-time PCR and the ability of multi-gene detection of microarrays.
Analyze the expression of a selected set of genes associated with a pathway or disease state.
A simple real-time PCR method has the advantages of qRT-PCR. High sensitivity and a wider dynamic quantitation range.
This integration allows for high specificity and amplification efficiency of PCR Arrays, while PCR chips are easy to use in any research laboratory.
PCR Arrays are sufficiently sensitive to use RNA prepared from routine samples (0.1–5 μg RNA), FFPE samples, and small samples (1–100 ng RNA).
The PCR Array system uses high-quality RNA to create a single band, no primer dimers or other secondary products at the desired location, resulting in highly accurate real-time PCR results.
The choice between sequencing and qPCR array depends on the number of samples, the total number of sequences in the target region, budget considerations, and research goals, etc.
qPCR array is usually a good choice when the number of target regions is small (≤20 targets) and the research goal is limited to screening or identifying known variants.
IntegrateRNA offers a variety of RNA qPCR array services, including sample quality checks, real-time PCR, and bioinformatics analysis. Finally, we will send you a service report that includes 1) a description of the materials and methods, and 2) a summary of the real-time PCR results. We also provide all raw data, normalized data and final output as an Excel file.