m5C RNA Bisulfite Sequencing Service

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m5C RNA Bisulfite Sequencing Service

In humans, 5-methylcytosine (m5C) is a rich RNA modification that is reported to be present in a variety of RNA species, including cytoplasmic and mitochondrial rRNA, tRNA, mRNA, enhancer RNA, and many non-coding RNAs. At least 10,275 m5C candidate sites were found in mRNA and ncRNA, covering 10.6% of total cytosine residues in the transcriptome. Recent data strongly suggest that RNA cytosine methylation affects the regulation of various biological processes including RNA stability and mRNA translation. In addition, m5C labeling is a versatile tool for fine-tuning RNA processing, stability, translation, and RNA-protein interactions.

Our understanding of m5C in RNA transcripts has only just begun to consolidate, but it is clear that cytosine methylation represents a powerful mechanism for regulating cellular processes at the level of apparent transcription.

IntegrateRNA is one of the first companies to provide m5C RNA methylation sequencing services., which use classic bisulfite processing and next-generation sequencing platforms to help you view the level of gene m5C methylation modification across the entire transcription range.

Project workflow

m5C RNA Bisulfite Sequencing Service

  • Step 1. Bisulfite treatment
    ➢ Submit your samples (including cells tissue, or dDNA), untreated or treated with bisulfite.
  • Step 2. Library construction
    ➢ Add adapters to ssDNA fragments (denatured by bisulfite treatment)
  • Step 3. Sequencing
    ➢ Sequence based on illumine platform
  • Step 4. Bioinformatics analysis
    ➢ Identify m5C sited, calculate methylation ratios, and perform differential methylation analysis

Strategy of m5C RNA bisulfite sequencing

m5C RNA Bisulfite Sequencing Service

Our features and benefits

  • One-stop service
  • ➢ We are able to process a variety of samples - Including total RNA isolated from a variety of tissue/cell samples such as fresh and frozen tissue.
    ➢ Offer optimized experimental scheme from extraction and bisulfite, and complete sequencing solution.

  • Processing to data analysis
  • ➢ High-sensitivity, high-efficiency experimental strategy - Optimized bisulfite method can eliminate fragment loss and selection bias, and improve the efficiency of cDNA library.

  • Strict quality control
  • ➢ IntegrateRNA has set up key quality control for each step of the m5C RNA-Seq experiment to ensure that customers get high-quality data.

  • Professional bioinformatics analysis
  • ➢ IntegrateRNA has a professional biological information analysis team to help customers conduct comprehensive mining of experimental data.

  • Professional Ph.D. support at every step, including delivery
  • Industry-leading turnaround times

As a leading biological commercial company specializing in RNA, IntegrateRNA provides comprehensive expert services to our clients for promoting various kinds of RNA discovery, functional analysis, bioinformatics analysis, and RNA therapy. One of our tasks is to provide partners with high-quality data in RNA modification/differential expression. Through a consulting, flexible, integrated and interdisciplinary approach, we provide advice, answers and services to advance your project progress. If you have any questions, please feel free to contact us.

For research use only. Not intended for any clinical use.
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