LC-MS based RNA Modification Analysis

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LC-MS based RNA Modification Analysis

As a leading biological commercial company, IntegrateRNA utilizes a state of the art, high performance LC-MS system to accurately detect and quantify the nucleoside modification of your total RNA, mRNA, rRNA, tRNA and other samples. Our standardized run contains the 40 most common modifications such as m6A, m5C, m3C, m7G or m1A. Other modifications can also be analyzed according to your requirements. Our mass spectrometry platform delivers a new level of sensitivity, precision, accuracy, dynamic range, and robustness of the quantification results.

RNA modification occurs in all living organisms, and is one of the most evolutionarily conserved properties of RNAs. Post-transcriptional modification of RNA is an important determinant of RNA quality control, translational efficiency, RNA-protein interactions, and stress response. RNA modification defects can affect the activity, localization as well as stability of RNAs, and has been linked with human diseases, such as cancers, neurological disorders (including ALS, PD), and mitochondrial-linked disorders (MELAS and MERRF). To elucidate the function and mechanism of RNA modification, IntegrateRNA utilizes advanced liquid chromatography-coupled mass spectrometry (LC-MS) platform to detect and quantify the modification of your RNA samples.

Workflow of RNA Modification Analysis

  • Total RNA preparation
  • tRNA or mRNA or rRNA purification (PAGE selection)
  • hydrolysis of RNA to individual ribonucleosides
  • LC-MS/MS: ribonucleosides identification and quantification
  • Data analysis:

We have flexible strategies for the detection and quantification of RNA modifications. Our highly experienced scientists execute quality management, following every procedure to ensure unbiased and precise results.

Option 1:

NucleosideSymbolNucleosideSymbolNucleosideSymbol
AdenosineA5-methyluridinem5U2'-O-methylguanosineGm
CytidineC3'-O-methyladenosine3'-OMeA3'-O-methylinosine3'-OMeI
GuanosineG1-methylpseudouridineM1Ψ2-thiouridines2U
UridineUN6-isopentenyladenosinei6A4-thiouridines4U
InosineI5,2'-O-dimethylcytidinem5Cm5-methyluridinem5U
N6-methyladenosinem6A5-hydroxymethylcytidinehm5CN4-acetylcytidineac4C
5-methylcytidinem5CN2,N2,7-trimethylguanosinem2,2,7G3'-O-methyluridine3'-OMeU
1-methyladenosinem1AN4-acetyl-2'-O-methylcytidineac4Cm5-methyl-2-thiouridinem5s2U
3-methylcytidinem3C3'-O-methylcytidine3'-OMeC5-methoxyuridineMo5U
1-methylguanosinem1G5,2'-O-dimethyluridinem5UmpseudouridineΨ
7-methylguanosinem7GN2,N2-dimethylguanosinem22G2'-O-methylinosineIm
3-methyluridinem3U5'-O-methylthymidineUm3'-O-methylguanosine3'-OMeG
2-thiocytidines2CN2-methylguanosinem2G2'-O-methyladenosineAm
2'-O-methylcytidineCm    

Option 2:

NucleosideSymbolNucleosideSymbolNucleosideSymbol
5'deoxyadenosine5'dAN 4-acetyl-2'-O-methylcytidineac4Cm5-iodocytidineI5C
2'-O-methyladenosineAmN 2-methylguanosinem2G5-carboxycytidineca5C
N 6-methyladenosinem6A1-methylguanosinem1GpseudouridineY
1-methyladenosinem1A2'-O-methylguanosineGm2'-O-mehtyluridineUm
8-hydroxyadenosine8-oxoA7-methylguanosinem7G5-methyluridinem5U
xanthosineXN 6,2'-O-dimethyladenosinem6Am3-methyluridinem3U
inosineIN6,N 6-dimethyladenosinem6,6A1-methylpseudouridinem1Y
2'-O-methylcytidineCmN6-(cis- hydroxyisopentenyl)adenosineio6A5-hydroxyuridines2U
3-methylcytidinem3CN6-threonylcarbamoyladenosinet6A4-thiouridines4U
5-methylcytidinem5C5-hydroxymethylcytidinehm5C5,2'-O-dimethyluridinem5Um
2-thiocytidines2CO 6-methylguanosinem6G5-hydroxymethyluridinehm5U
5-formylcytidinef5cN 2,N 2-dimethylguanosinem2,2G5-methoxycarbonylmethyl-2-thiouridinemcm5s2U
5,2'O-dimethylcydinem5Cm7-methyl-6-thioguanosinem7s6G5-methoxyuridinemo5U
N4-acetylcytidineac4CN2,N 2,7-trimethylguanosinem2,2,7G5-methyl-2-thiouridinem5s2U
6-methyluridinem6U2-thio-2-methyluridines2UmN6-acetyladenosineac6A
N4-methylcytidinem4C5-methoxycarbonylmethyuridinemcm5UN4,2'-O-dimethylcytidinem4Cm

Option 3:

NucleosideSymbolNucleosideSymbolNucleosideSymbol
N 6-methyladenosinem6A2'-O-methylcytidineAm2'-O-methyluridineUm
5-methylcytidinem5C2'-O-methylcytidineCm2'-O-methylguanosineGm

Benefits

  • Full service — from sample preparation to results analysis
  • Best comprehensive analysis — coverage 40 nucleoside modifications in RNA
  • National advanced laboratory, professional team and efficient operation
  • High Performance — highly optimized experimental procedures, state-of-the-art LC-MS system, expertise in operation.

IntegrateRNA combines the separation capabilities of high-pressure liquid chromatography (HPLC) and the mass analysis capabilities of mass spectrometry to provide very sensitive and specific detection of RNA molecules. We offer a set of LC-MS-based RNA modification analysis services to accurately determine the identities and concentration of RNA modifications in your sample. To learn more about our capabilities for RNA modification analysis and to determine feasibility for your project, please contact us at or 1-631-626-9181.

For research use only. Not intended for any clinical use.
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