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Specializing circRNA field for many years, IntegrateRNA offers one stop solution of circRNA research, including but not limited to circRNA expression profiling analysis, screening, identification, function analysis and high-throughput sequencing service. Now we develop a series of circRNA translation-related vector to help clients validate whether the circRNAs are indeed translatable, using experimental approaches. We will assist you to investigate different aspects of circRNA biogenesis and to gain insights into mechanisms of circular RNA translation.
Mounting evidence have shown that circRNAs can be associated with polysomes and some of them comprise the initiation codon AUG and putative Open Reading Frames (ORF) with favorable length, which suggests an unexpected protein-coding potential for circRNAs. Actually, circRNAs can encode regulatory peptides, and a hidden proteome encoded by circRNAs might exist. The circular structure of circRNAs necessarily implies a cap-independent and internal mechanism to initiate translation. One possibility in the cells, for cap-independent translation initiation, is the presence of an IRES (Internal Ribosome Entry site) structure in the 5' UTR of mRNAs. Another mechanism is the cap-independent translation initiation under stress through methylated adenosine residues in the form of N6-methyladenosines (m6A) in the 5' untranslated region (5'UTR) of some transcripts. Thus, Internal Ribosome Entry site (IRES)- and N6-methyladenosines (m6A)-mediated cap-independent translation initiation are potential mechanisms for circRNAs translation.
Figure 1. Cap-independent translation initiation mechanisms of circRNAs
IntegrateRNA offers circRNA ORF/IRES prediction and annotation service using a series of advanced bioinformatics algorithms. Our service assist you accurately and quantitatively assess the coding potential of your circRNA, as well as provide insights into the function and mechanisms of circRNA-derived proteins.
After computational assessments, it is desirable to experimentally validate whether the circRNAs are indeed translatable. Ribosome profiling and Ribosome nascent-chain complex sequencing enable translational global analysis. Moreover, IntegrateRNA develops a series of ORF/IRES validation vector of circRNA, which can be applied in circRNA translation evaluation and mechanism analysis.
Figure 2. Plasmids structure of circRNA ORF/IRES validation
IntegrateRNA offers circRNA translation prediction and evaluation service, by bioinformatics algorithms, Ribosome profiling and experimental approaches. We are dedicated to provide complete, innovative solutions to help you solve the problems you encounter during your research. For more information, please feel free to contact us.