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siRNA is produced by cleavage of dsRNA templates by DICER, an RNAse III ribonuclease. The siRNA can be incorporated into an RNA-induced silencing complex (RISC) and unwound into single-stranded siRNA. Next, single-stranded siRNA directs the RISC complex to the target mRNA for disruption, causing RNA interference. Depending on the amount of siRNA expressed and its inhibition efficiency, expression of the target gene can be completely blocked or measurably inhibited. This allows researchers to identify and study the function of genes, especially those that are lethal after complete knockout.
The siRNA enters the living host cell by the following method:
Vector-based siRNAs have stronger in vivo inhibition than synthetic siRNAs, which are rapidly processed into active siRNAs in cells and can work in concert with tissue-specific promoters. Since stable cell lines can be developed, vector-based siRNA allows researchers to observe long-term knockdown effects of their target genes.
IntegrateRNA is committed to providing a wide range of RNA products and solutions for efficient and specialized RNA researchers. Our vector-based siRNA construction service can help you identify the most potent siRNA sequences with powerful gene silencing capabilities and clone them into any cloning vector you want.
As a leading biotechnology company, IntegrateRNA has many years of experience in RNA research. We are committed to providing the most reliable and effective services to help you solve the problems you encounter. Tell us the genes you are interested in, we will design 3-5 target sites, or you can provide specific siRNA target sequences, and deliver the final vector-based siRNA within shorter turnaround period, which will have been confirmed by sequencing. There is no doubt that IntegrateRNA is the best partner during your research. If you have any questions, please feel free to contact us.