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siRNA Services

What is siRNA?

siRNA (small interfering RNA), also named as short interfering RNA or silencing RNA, is a class of double-stranded RNA molecules (usually 20 to 24 bp). Structurally, siRNA has phosphorylated 5' ends and hydroxylated 3' ends with two overhanging nucleotides. The Dicer enzyme catalyzes the production of siRNA from long dsRNA and small hairpin RNA. The siRNA operates within the RNA interference (RNAi) pathway, which interferes with the expression of particular genes with complementary nucleotide sequences by post-transcriptional degradation of mRNA, thereby preventing translation.

Mechanism of RNAi

  • Dicer (RNase III-like enzyme) cleaves longer double-stranded RNA (dsRNA) to form siRNA.
  • The siRNA binds with other proteins to form the RNA-induced silencing complex (RISC).
  • Within the RISC complex, the siRNA strands are separated and the more stable 5'-end strand is typically integrated into the active RISC complex.
  • As part of the RISC complex, single-stranded siRNA scans and finds complementary mRNA, and then induces mRNA cleavage once the single-stranded siRNA binds to its target mRNA.

Figure 1: Mechanism of gene knockdown  by siRNAs (Anders Wittrup; 2015)Figure 1: Mechanism of gene knockdown by siRNAs (Anders Wittrup; 2015)

Great potential for diseases therapy

siRNA can be used as a tool for studying single gene function in vivo and in vitro, and siRNA has attracted attention as a potential therapeutic agent due to its ability to inhibit specific genes in many diseases. In particular, it is an unstoppable target for the treatment of cancer and other diseases. siRNA therapy is now ready to enter the clinical formulary and is going to be a new class of drugs in the near future. siRNA-based therapies are becoming a promising new anticancer approach, and a small number of clinical trials involving solid tumor patients now are programing.

As one of the global biotechnology companies specializing in RNA research, IntegrateRNA is dedicated to providing products and services to a wide range of genomics researchers in the market, including various types of RNA discovery, analysis, and related bioinformatics services, which have fully recognized by our clients. To support your projects, our team of siRNA experts provides complete, innovative solutions and high-quality products to help you solve the problems you encounter during your research. We guarantee our clients the most reliable and efficient research services to best match your research goals. If you have any questions, don’t hesitate to contact us.

References:

  • Hassan Dana. et al. Molecular Mechanisms and Biological Functions of siRNA. Int J Biomed Sci. 2017 Jun; 13(2):48–57.
  • Ghanbar Mahmoodi Chalbatani. et al. Small interfering RNAs (siRNAs) in cancer therapy: a nano-based approach. Int J Nanomedicine. 2019; 14:3111–3128.
  • Anders Wittrup and Judy Lieberman. Knocking down disease: a progress report on siRNA therapeutics. Nat Rev Genet. 2015; 16(9):543–552.
For research use only. Not intended for any clinical use.
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