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piRNA Array Service

piRNAs are a class of non-coding RNAs of approximately 26-32 nucleotides in length. The piRNA interacts with the PIWI (P-element-induced Wimpy Testis, subfamily of the Argonaute protein) to form a piRNA/PIWI complex, thereby silencing the transposable element to maintain genomic integrity, as well as phylogeny and spermatogenesis. The piRNA is produced by a primary transcript that traverses the entire piRNA cluster and is subsequently processed into mature piRNA. Although piRNA has been found in large numbers in many species, there are many unresolved problems in the field of piRNA that need further study.

IntegrateRNA is one of the world's leading biotechnology companies specializing in RNA research, providing products and services to a wide range of genomic researchers on the market, including various types of RNA discovery, analysis, and related bioinformatics services. Based on our platform, we are able to offer you with a new piRNA Arrays service designed to analyze tens of thousands of piRNAs with high specificity and sensitivity for piRNA biology, molecular function and biomarker applications.

Workflow of piRNA array Service

  • RNA isolation (optional)
  • RNA QC
  • The absorbance of RNA at 260 nm, 280 nm, and 230 nm of the spectrophotometer is measured to calculate the concentration and evaluate the purity
  • Denaturing agarose gel electrophoresis is carried out with formaldehyde electrophoresis reagent to detect RNA purity and integrity
  • Provide RNA QC reports

Note: RNA samples used for chip detection must be of high quality, intact, free of RNase contamination (degraded samples cannot be used for labeling and chip detection) and are free of genomic contamination.

  • Target repair with Cy3 tag
  • Array hybridization, cleaning and scanning
  • Data extraction, analysis and summary
  • Experimental reports - including detailed experimental methods and chip experiment data and charts
  • Chip scan
  • Operating instructions (including RNA quality inspection report)
  • Chip data
  • Data summary table (EXCEL format, including probe position, probe name, original signal value, corrected signal value, normalized signal value, the ratio of piRNA expression between samples)
  • Data table for differential expression of piRNA (EXCEL format, including piRNA with up-regulated expression of 2 fold and piRNA with down-regulated expression by 2 fold)
  • Further data analysis
  • Scatter Plot (mainly for correlation R-value calculation for monochrome repeated experimental data)
  • Hierarchical clustering (multiple samples are grouped according to gene expression similarity when multiple sample experiments are performed)

If repeating the experiment (≥3), calculate the CV value, p-value, and do Volcano Plot

Benefits:

  • Comprehensive collection and reliable selection of piRNA sequences
  • A 60-mer oligonucleotide probe designed in a double strand was used to achieve high specificity
  • Repeat probes for most piRNAs

IntegrateRNA, with many years of experience in RNA research, is dedicated to providing comprehensive RNA products and services for academic research, biotechnology research and drug discovery. With skilled and experienced genomics experts, we are able to provide our clients with professional and efficient piRNA array services to help you accurately profile the expression of piRNAs. We are committed to providing the most reliable and effective research services to best meet your research goals. If you have any questions, please feel free to contact us.

For research use only. Not intended for any clinical use.
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