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IntegrateRNA provides you with mRNA sequencing services using state-of-the-art Illumina platforms, with superior data quality and accurate sequence assembly. Our comprehensive solution including sample preparation/QC, library construction, deep sequencing, raw data quality control, and bioinformatics analysis. Our experienced bioinformaticians work closely with customers to provide standard and customized data analysis and publication ready results, for any species of interest.
mRNA is a large family of the intracellular RNA molecules that convey genetic information from DNA to the ribosome. They are specific at the amino acid level of the protein products of gene expression. Analyzing mRNA transcriptome has rapidly become the method of choice for analyzing the transcriptomes of disease states, of biological processes, and across a wide range of study designs. It is both a highly sensitive and accurate method for quantifying gene expression. Since mRNA accounts for only 1-4% of the entire transcriptome, we enriched the transcript by the poly(A) tail of the mRNA when preparing the library to increase the depth of sequencing of the encoded mRNA and sensitivity to the low-level expression of mRNA.
We provide you with RNA extraction services that separate RNA from samples for sequencing. We perform high-quality RNA isolation from a range of samples including cells, tissues, FFPE sections, blood, plasma, and exosome.
IntegrateRNA provides researchers with accurate and complete transcriptome information that not only recognizes new transcripts, alternative splicing, and gene fusion events but also quantifies gene expression and differential gene expression analysis between different sample groups. We can tailor mRNA data analysis pipeline to your research interest. If you have additional requirements or questions, please feel free to contact us.