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IntegrateRNA provides custom miRNA qPCR array analysis service to a wide range of researchers for high-throughput profiling of miRNA expression, which is based on a highly sensitive and specific miRNA qPCR system. Our qPCR array services are designed for expression analysis of cancer-related, pathway-related and whole genome miRNAs, and can be applied in biomarker discovery and therapy development research of miRNA.
MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression at the post-transcriptional level. Usually 21-23 nucleotides in length, miRNAs are important modulators in cellular pathways. The miRNA expression analysis is necessary to gain understanding of the role of miRNA in disease pathogenesis or cellular pathways as well as to identify or validate key markers. IntegrateRNA’ qPCR arrays are designed to analyze predefined or customized miRNA expression sets in various tissues or cells. The differential expression result from heterotypic miRNAs can help researchers identify miRNAs of biological significance and importance associated with research.
Figure1. miRNA RT-PCR mechanism
As shown, we use cutting-edge technology to integrate universal tailing and reverse transcription with miRNA specific PCR. The assay is highly sensitive, up to 10 miRNA copies can be detected.
➢ Reverse transcription control – monitors the efficiency of the RT response.
➢ Positive PCR control – verify PCR efficiency.
➢ The housekeeping gene – can be used as endogenous positive control and used for array standardization.
➢ Negative control – can be used as a negative PCR control.
IntegrateRNA has long term devoted to the field of varieties of RNA discovery and bioinformatics analysis service. We provide sensitive miRNA qPCR arrays for profiling of mature miRNAs in cancers/specific pathways or for screening the whole miRNome (covering the complete miRbase). Our expert scientists perform your miRNA expression analysis in our state-of-the-art automated labs with rigorous quality control and maximum repeatability according to your research needs and budget.