miRNA qPCR Array Service

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miRNA qPCR Array Service

IntegrateRNA provides custom miRNA qPCR array analysis service to a wide range of researchers for high-throughput profiling of miRNA expression, which is based on a highly sensitive and specific miRNA qPCR system. Our qPCR array services are designed for expression analysis of cancer-related, pathway-related and whole genome miRNAs, and can be applied in biomarker discovery and therapy development research of miRNA.

MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression at the post-transcriptional level. Usually 21-23 nucleotides in length, miRNAs are important modulators in cellular pathways. The miRNA expression analysis is necessary to gain understanding of the role of miRNA in disease pathogenesis or cellular pathways as well as to identify or validate key markers. IntegrateRNA’ qPCR arrays are designed to analyze predefined or customized miRNA expression sets in various tissues or cells. The differential expression result from heterotypic miRNAs can help researchers identify miRNAs of biological significance and importance associated with research.

miRNA qPCR Array ServiceFigure1. miRNA RT-PCR mechanism

As shown, we use cutting-edge technology to integrate universal tailing and reverse transcription with miRNA specific PCR. The assay is highly sensitive, up to 10 miRNA copies can be detected.

Key Advantages

  • Proven primers – each primer is designed using a proprietary algorithm and has been experimentally verified.
  • Flexible array design – offer a variety of layouts to choose from, including 96-well and 384-well plates.
  • Specificity – ability to distinguish miRNAs with single nucleotide mismatches.
  • Extensive linearity – simultaneous detection of miRNAs at various expression levels.
  • Reproducible – high reproducibility of inter-array and intra-array replication (R2 > 0.99).
  • Whole-genome coverage, prearranged groups or customized groups.
  • Different controls can also be included in the array to help monitor sample quality

➢ Reverse transcription control – monitors the efficiency of the RT response.

➢ Positive PCR control – verify PCR efficiency.

➢ The housekeeping gene – can be used as endogenous positive control and used for array standardization.

➢ Negative control – can be used as a negative PCR control.

Applications

  • Pathway-focused and disease-focused miRNA profiling
  • miRNome profiling
  • Biomarker discovery

IntegrateRNA has long term devoted to the field of varieties of RNA discovery and bioinformatics analysis service. We provide sensitive miRNA qPCR arrays for profiling of mature miRNAs in cancers/specific pathways or for screening the whole miRNome (covering the complete miRbase). Our expert scientists perform your miRNA expression analysis in our state-of-the-art automated labs with rigorous quality control and maximum repeatability according to your research needs and budget.

For research use only. Not intended for any clinical use.
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