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IntegrateRNA, a leading global biotechnology company specializing in RNA research, recently introduced the latest optimized miRNA pull-down strategy to help you identify the direct interactions between miRNAs and their target mRNAs in a faster and more accurate way. Our experienced team can provide flexible solutions for any individual request.
MicroRNAs (miRNA) are small non-coding regulatory RNAs, controlling key biological processes in a broad range of eukaryotic cells. The predominant function of miRNAs is to act as guide molecules in the RNA-induced silencing complexes (RISC) and target regions of complementarity in the mRNAs. Although bioinformatic tools have been employed to predict sequences within the 3' UTR of mRNAs as potential targets for miRNA binding. These computational methods often generate false positive results and are limited to predicting canonical interaction between miRNA and mRNA. Therefore, experimental procedures that measure direct binding of miRNA to its mRNA target are necessary to establish functional interaction. miRNA pull-down is a powerful tool to identify miRNA targets.
As shown, we use biotinylated miRNA mimics as bait to enrich miRNA-mRNA complex, and streptavidin-coated magnetic beads absorb and screen the complex, which is more conducive to direct capture than antibody enrichment.
IntegrateRNA has many years of experiments in RNAs research and is dedicated to providing a comprehensive range of various types of RNA products and services for academic research, biotechnology research, and drug discovery. Based on the powerful platform and advance technique, we provide miRNA pull-down assay service to help you reveal the interaction mechanism between mRNA and miRNA. We guarantee our clients the most reliable and efficient research services to best match your research goals with faster turnaround time, lower prices and more comprehensive data analysis. If you have any questions, please feel free to contact us.