miRNA Sequencing Service

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miRNA Sequencing Service

IntegrateRNA is a leading global biotechnology company, and is dedicated to providing a wide range of services for RNA discovery, analysis, and related bioinformatics analysis. Our experts have examined thousands of different RNA data sets, which gives us a unique insight into RNA research. To support your research, we offer complete microRNA sequencing services including RNA preparation and quality control, library construction, sequencing, and comprehensive data analysis, producing the results needed to rapidly advance biological and biomedical research.

MicroRNAs (miRNA) are small, non-coding RNA molecules that function broadly as negative regulators of gene expression to control a wide range of cellular processes. Thousands of miRNAs have been identified in humans, but only a small fraction of them have demonstrated functional roles. Among them, miRNAs involving human diseases, especially cancer, have attracted great interest. With the discovering of new microRNAs and the uncovering of their importance various biological functions as small regulatory elements, miRNA research is rapidly evolving. miRNA sequencing is used to discover novel miRNAs, examine the dierential expression of all miRNAs and to characterize variations with single-base resolution.

Workflow of miRNA Sequencing Service

miRNA Sequencing Service

Sample Requirements

  • Sample type: Total RNA without degradation or DNA contamination
  • Starting amount of total RNA ≥ 5 µg
  • Sample concentration ≥ 200 ng/µl
  • Sample purity: OD260/280 = 1.8~2.2

Bioinformatics Analysis Pipeline

miRNA Sequencing Service

In-depth miRNA Data Analysis

  • Quality control of raw data
  • miRNA classification and annotation
  • Analysis for known miRNA (including expression levels, length distribution, and base preference)
  • Prediction for novel miRNA and miRNA target gene
  • Analysis of the differential expression of novel miRNA
  • Correlation analysis between differentially expressed miRNA and differentially expressed target genes
  • Interaction network between differentially expressed miRNA and differentially expressed target genes
  • Functional clustering analysis of interaction target genes (KEGG and GO)

Benefits

  • Comprehensive bioinformatics data analysis using widely accepted mainstream software and proven internal pipelines to discover novel microRNA, target gene prediction, differential expressions and functional annotate (gene ontology/pathway analysis), etc.
  • Expert technical support to assist with data interpretation
  • Data visualization and publication-quality graphics
  • Highly optimized program to maximize mappable readings and provide accurate quantification

IntegrateRNA, a biotechnology company with experienced scientists and advanced technology, is dedicated to providing the most comprehensive and professional services for microRNA sequencing and analysis. Based on rigorous quality control system and advanced sequencing platform, we will deliver accurate, affordable and high-quality sequencing data to support your research. Don’t hesitate to contact us, we guarantee our clients the most reliable and efficient research services.

For research use only. Not intended for any clinical use.
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