miRNA Microarray Service

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miRNA Microarray Service

IntegrateRNA uses the most powerful microarray platforms to provide you with the best miRNA expression profiling services that are constantly being validated, tested and updated. We have standard and custom microarrays for all mature miRNAs of all species available in miRBase V21.0 database. Our comprehensive service includes sample control, sample preparation and labeling, microRNA detection, array scanning, data extraction, and full data analysis. IntegrateRNA is confident to provide the most affordable, high-quality custom miRNA microarray service in a short turnaround time.

MicroRNAs (miRNAs) are small single-strand non-protein-coding RNA molecules, of 17-25 nucleotides in length. miRNAs function as negative posttranscriptional gene regulators, regulating mRNA translation and degradation in animals, plants and viruses. Recent evidences demonstrated that miRNAs play a critical role in many biological processes, also including haematopoietic cell differentiation, apoptosis, cell proliferation, organ development and stress response. Thus, it becomes a trend to understand the miRNA and mRNA expression profiles simultaneously using a holistic approach.

Workflow of miRNA Microarray Service

miRNA Microarray Service

Recommendations

Table1. miRNA expression microarrays

MicroarrayFormatsDetected miRNADatabase
Agilent Human miRNA Microarray V21.08*60K2,549 miRNASanger miRBase V21.0
Agilent Mouse miRNA Microarray V21.08*60K1,881 miRNASanger miRBase V21.0
Agilent Rat miRNA Microarray V21.08*15K759 miRNASanger miRBase V21.0
Agilent Custom miRNA MicroarrayInquireInquireSanger miRBase V22.0
Affymetrix GeneChip miRNA V4.02/6/30 arrays30,424 mature miRNA (all organisms), 1,996  snoRNA and scaRNASanger miRBase V20.0, Ensembl, snoRNAbase, mirTarbase

Bioinformatics Analysis

  • Data quality control
  • Differential expression miRNA screening
  • Gene targets predict differentially expressed miRNAs
  • Gene ontology and path enrichment analysis for predicting gene targets
  • miRNA-lncRNA/circRNA/ transcription factor interaction network analysis
  • ceRNA (competing endogenous) network analysis
  • Further customization of bioinformatics analysis to suit your unique project

Features and Benefits

  • Comprehensive coverage: designed to interrogate all mature miRNA sequences in miRBase V21.0.
  • Easily correlate miRNA results: analysis files contain host gene ID, predicted and validated miRNA target genes, and clustered miRNA information
  • Handling various research and clinical sample types: including tissue, cell, FFPE, urine, blood, plasma, exosome samples.

Application

  • Conducting a genome-wide analysis of miRNA expression of normal and disease samples
  • Discovering the potential biomarkers and therapeutic targets
  • Identifying the miRNA involved in the pathogenesis of human cancers

IntegrateRNA offers genome-wide miRNA expression profiling service using a microarray detection system developed specifically for miRNA detection. We can also provide you with the most affordable and high-quality custom miRNA microarray service. For more information, don’t hesitate to contact us.

For research use only. Not intended for any clinical use.
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