LncRNA Sequencing Service



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LncRNA Sequencing Service

IntegrateRNA's experts have examined thousands of different RNA data sets, which gives us a unique insight into RNA research. To support your research, we use next-generation sequencing technology and bioinformatics analysis to provide you with one-stop lncRNA-seq service from RNA preparation to final comprehensive bioinformatics data analysis, rapidly advancing the results required for biological and biomedical research.

LncRNA (long non-coding RNA) is a class of RNA molecules longer than 200 nucleotides, similar to mRNA, as they are usually transcribed, polyadenylated and capped by reactive chromatin. However, lncRNA do not direct protein synthesis. LncRNA is a relatively abundant component of mammalian/plant transcriptomes and is involved in several cellular functions including nucleosome relocalization, chromatin remodeling, transcriptional control, and post-transcriptional processing. Recent studies show that lncRNA is increasingly involved in genomic imprinting, developmental regulation, and plays a key role in a variety of human diseases, including cancer, cardiovascular disease, and neurological diseases. LncRNA sequencing helps to reveal complete images of lncRNA in biological samples under specific conditions and to understand their role in many human diseases.

Workflow of LncRNA-seq in IntegrateRNA

LncRNA Sequencing Service

Sample Requirements

  • RNA sample concentration ≥100 ng/μL
  • RNA sample amount ≥3 μg
  • Purity: OD260/280 ≥1.8, OD260/230 ≥0.5, RIN ≥7.0, 28S/18S ≥1.0

Sequencing Strategy

  • 250-300 bp insert directional cDNA library with rRNA removal
  • Illumina platform, paired-end 150 bp

Recommended Sequencing Depth

  • 12 G raw data for normal-depth sequencing
  • 24 G raw data for high-depth sequencing

Bioinformatics Analysis Pipeline

LncRNA Sequencing Service

In-depth lncRNA Data Analysis

  • Predict new transcripts (including lncRNAs)
  • Comparison of basic properties of lncRNA and mRNA
  • Quantitative and differential expression analysis of lncRNA and mRNA
  • Localization of lncRNA on the genome
  • Classification of lncRNA family and annotation of lncRNA function
  • SNP/Indel call to identify splice variants
  • LncRNA target prediction and lncRNA-mRNA interaction analysis

Advantages of LncRNA Sequencing

  • Identifies known and novel features
  • Allows profiling of lncRNAs across a wide dynamic ranges
  • Explores novel biomarkers and lncRNAs regulatory networks

With experienced scientists and advanced technology, IntegrateRNA is dedicated to providing the most comprehensive and professional services for lncRNA sequencing and analysis. Tell us your needs, we can help you get single-base resolution lncRNA sequence information in one go, through rigorous quality control and advanced bioinformatics analysis. Don't hesitate to contact us, we guarantee our clients the most reliable and efficient research services.

For research use only. Not intended for any clinical use.
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