LncRNA Pull-down Assay

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LncRNA Pull-down Assay

Based on extensive experience in the lncRNA field, IntegrateRNA provides customers with a comprehensive lncRNA pull-down service, from the initial experimental design to the identification of the last interacting molecules. Our experienced scientists and advanced technologies enable us to provide consistent and high-quality data in a cost-effective manner.

Studies have shown that lncRNA has a conserved secondary structure, can interact with proteins, DNA and RNA, and participates in a variety of biological processes. With the development of high-throughput sequencing technology, more and more lncRNAs have been annotated, but the functions of most lncRNAs are still unclear. Therefore, the study of lncRNAs is a very broad unknown field and has great scientific value. RNA pull-down technology has played a vital role in the validation of lncRNA biology.

High-throughput screening for the interacting proteins of lncRNA is convenient by pull-down combined with LC-MS. IntegrateRNA utilizes LC-MS to identify captured protein complexes, and then screen specificity Interacting proteins with the target lncRNA.

Strategy of lncRNA pull-downFigure1. Strategy of lncRNA pull-down

Project Workflow

  • Consult our lncRNA group engineering design experts to establish the exact lncRNA pull-down strategy
  • In vitro transcription of labeled lncRNA
    ➢ Clone the lncRNA sequence and construct related vectors
    ➢ Biotin-labeling RNA in vitro transcription
    ➢ Purification
  • Cell lysate preparation
  • Activation of beads
  • LncRNA pulldown
  • Incubation
  • Wash out
  • SDS-PAGE, MS analysis, etc.

Deliverables

  • Sequencing report and plasmid map of vectors used in this project
  • Sensing & antisense primers and template electrophoresis images of in vitro transcription templates
  • Mass spectrometry report

Our Features and Advantages

  • Extensive RNA system expertise and application experience
  • Efficient lncRNA pull-down design strategy to ensure the most effective efficiency
  • Unique technology platform ensures shorter turnaround times and lower prices
  • The final report with detailed project process and quality monitoring data

IntegrateRNA is a professional biotechnology company specializing in RNA expression analysis and RNA interaction molecules to explore the function of RNA in the development of human diseases and their potential applications in therapy. Our experienced scientists and advanced technologies enable us to deliver consistent and high-quality data with cost-efficiency. If you have any questions or requirements, please feel free to contact us.

For research use only. Not intended for any clinical use.
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