LncRNA Knockout Service

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LncRNA Knockout Service

IntegrateRNA has successfully implied CRISPR/Cas9 system into loss-of-function studies of lncRNA. Recently we have developed custom lncRNA knockout service via CRISPR/Cas9 system to help you knockout lncRNAs you are interested in cells. We also utilized CRISPR/Cas9 to construct lncRNA library for large-scale screening of functional lncRNA in multiple cell lines. Based on excellent platform and leading-edge technology, our experienced scientist team serves our customers with high-quality services.

LncRNAs are defined as the noncoding linear transcripts longer than 200 nucleotides, which play important roles in the regulation of transcription and post-transcriptional processes of coding and non-coding RNAs. Moreover, lncRNAs have been found to function as novel types of oncogenes and tumor suppressors during cancer progression through various mechanisms, which endow them with the potential of serving as reliable biomarkers and novel therapeutic targets for cancers. For many lncRNAs with complex transcriptional profile and regulatory regions, the CRISPR/Cas9 mediated ablation of the locus can act as a very specific and powerful tool to study their functions both in vitro and in vivo.

Strategy of LncRNA Knockout

Strategy of LncRNA Knockout

As shown, Cas9 can be directed by a pair of specifically designed sgRNAs targeting the 5' and 3' ends of the lncRNA gene to generate lncRNA fragment deletion. This method is particularly useful for performing CRISPR lncRNA knockout, as simple indel mutations do not facilitate effective knockout of lncRNA. Besides, we also utilize a paired-guide RNA library to achieve whole genome-scale delection of lncRNAs. This is convenient for high-throughput screening of lncRNA knockout phenotypes.

Workflow of LncRNA Knockout Service

  • sgRNA design and construction of 2 sgRNA pairs in CRISPR vector
  • HDR template synthesis and construction (optional)
  • Cell culture, transfection, optimization
  • Clonal selection and screening: knockout confirmation by PCR or Sanger sequencing
  • Clonal expansion and cryopreservation

Application

  • LncRNA function research
  • Discover new lncRNAs in various human diseases (e.g. nervous system diseases, cancer, etc.)

IntegrateRNA, a world-leading biotechnology company, is dedicated to providing the most comprehensive range of specialized RNA products and services to genetic researchers for basic research, drug development, and clinical research. Tell us your needs, we will offer you a comprehensive lncRNA knockout service from strategy design to final stable cell line. We guarantee our clients the most reliable and efficient research services to best match your research goals with shorter turnaround time and lower price. If you have any questions, please feel free to contact us.

Reference:

  1. Li, L. et al. (2014). Physiological roles of long noncoding rnas: insight from knockout mice. Trends in Cell Biology, 24(10), 594-602.
For research use only. Not intended for any clinical use.
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