Custom tRNA Synthetase Service

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Custom tRNA Synthetase Service

tRNA synthetase - enzyme is also named aminoacyl-tRNA synthetase (aaRSs). In the first step of protein synthesis, each enzyme can exquisitely link the respective amino acid to its cognate tRNA isoform. This diverse set of proteins is united by a common aminoacylation reaction, which attaches an amino acid to its cognate tRNA. In humans, 20 different types of aa-tRNA are composed of 20 different aminoacyl-tRNA synthetases, each corresponding to each amino acid in the genetic code. There are two classes of tRNA synthetases divided based on their chemical properties, architecture of their catalytic domains and consensus sequences.

Table 1: Classes of tRNA synthetases (Michael Ibba, 2000)

Class ILigandsClass IILigands
Subclass IaSubclass IIa
LeuRS SerRStRNA, Ser-AMP, ATP, Ap4A, analog
IleRSIle,ValThrRStRNA
ValRS ProRStRNA
MetRSATPHisRSHis-AMP, histidine, analog
CysRSCys, ATPGlyRSGly-AMP, analog
ArgRSArgSubclass Ilb
Subclass IbAspRStRNA, Asp-AMP, ATP,analog
GlnRSATP, tRNA, analogAsnRSATP, analog
GluRS LysRS-IItRNA, lysine
LysRS-ISubclass Ilc
Subclass IcPheRStRNA, Phe-AMP, Phe
TyrRSTyr, Tyr-AMP, analogAlaRS
TrpRSTrp-AMPGlyRS 

These tRNA synthetases control gene expression within the cell of origin, and when released exert homeostatic and developmental control in specific human cell types, tissues and organs during adult or fetal development or both. Non-translational functions of tRNA synthetases, affecting the pathways for cardiovascular development, immune response and mTOR, IFN-γ and p5 signaling, have recently been discovered. And those new functions play an important role in the stability of the intracellular environment (Figure 1). The associations of tRNA synthetases with specific diseases has also stimulated investigations of disease mechanisms. Since they are essential, the tRNA synthetases have become drug targets for the development of drugs and antibiotics. In recent years, the discovery of important new functions of this protein family has provided new and promising ways to treat the development of various human diseases.

Figure 1: The non-translational functions of tRNA synthaseFigure 1: The non-translational functions of tRNA synthase

IntegrateRNA is a leading biotech company specialized in RNA research, providing a comprehensive range of premium quality tRNA related services. All tRNA synthases products have undergone strict quality control, which are independently developed by our professional scientist team. We will provide you with high quality custom services. Our services include, but is not limited to:

  • Synthesis of tRNA synthetases gene services

According to your needs, we provide tRNA synthetase gene services of different species including Human, Mouse, Rat, Rabbit, Zebrafish, Drosophila etc.

  • Custom tRNA synthetases recombinant vector

Suitable for cell transfection, protein induction and purification, fusion tag as GST/His/GFP/myc/HA/ is available.

  • qPCR services

Design and provide forward and reverse primers for analyzing the quantitative expression of gene according to your project needs.

If you don’t see the tRNA synthetase services you need above, or to learn more about our capabilities for custom tRNA synthases products and to determine feasibility for your project, please contact us. Based on national advanced laboratory platforms, professional team and efficient operation, we guarantee our clients the most reliable and efficient research services to best match their research goals.

References:

  1. Michael Ibba. et al. AMINOACYL-TRNA SYNTHESIS. Annu. Rev. Biochem. 2000. 69:617–50.
  2. Min Guo & Paul Schimmel. Essential Non-Translational Functions of tRNA Synthetases. Nat Chem Biol. 2013; 9(3):145–153.
  3. Yan Ling. et al. tRNA synthetase: tRNA Aminoacylation and beyond. Wiley Interdiscip Rev RNA. 2014; 5(4):461–480.
  4. Vijayakumar Rajendran. et al. Aminoacyl-tRNA synthetases: Structure, function, and drug discovery. International Journal of Biological Macromolecules. 2018; 111:400-414.
For research use only. Not intended for any clinical use.
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