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Custom shRNA Lentivirus Services

shRNA is a type of synthetic double-stranded small RNA that silences target gene expression by RNAi. shRNA is a favorable RNAi medium because of its relatively low degradation and conversion. shRNAs expressed in cells are typically delivered by a plasmid, bacterial or viral vectors. By using co-RNAi, multiple shRNA delivery strategies can improve gene knockdown, allowing simultaneous targeting of multiple transcripts and reducing the appearance of viral escape mutants.

IntegrateRNA, one of the global biotechnology companies specializing in RNA research, is dedicated to providing a comprehensive range of RNA solutions, products, and services for academic research, biotech research and pharmaceutical drug discovery. To support your project, our shRNA expert team provides you with custom ready-to-use shRNA lentivirus for any specific gene to help you solve problems encountered during your research.

Our services include, but are not limited to

  • Constitutive or inducible version of the H1 or U6 shRNA promoter
  • Alternative markers: GFP, RFP, PuroR, BleoR, NeoR or Hygro-HK, etc.
  • If you provide a knockdown sequence, we only need to make shRNA DNA oligo-duplex and clone it into the shRNA vector
  • If you still can't provide a valid knockdown sequence, we will design a set of 3 shRNA constructs. We ensure that at least one of these three shRNA constructs shows ≥75% of target RNA, and we provide a free negative control shRNA lentivirus

Schematic representation of shRNA expression lentivirus vectors:

Custom shRNA Lentivirus Services

For shRNA expression, you can choose the constitutive U6 promoter or the inducible H1 promoter.

In order to take advantage of the inducible properties of the promoter, the repressor protein TetR must be present. Expression of TetR can be achieved by using our pre-prepared Tet-repressor lentiviral particles or by using our stable Tet-repressor cell line. In the presence of TetR, H1-driven shRNA transcription is blocked and expression can be induced by the addition of tetracycline.

Why choose us?

  • Safe use (self-inactivation) lentiviral particles can deliver your genes to a wide range of cell lines, including non-dividing, primary or stem cells.
  • Our lentiviral vectors efficiently integrate genes into early cell genomes.
  • The strongest promoter, the highest protein expression in mammalian cells.
  • Inducible or constitutive expression can be selected.
  • High titer of lentivirus, monitored by fluorescent protein (not fused to target).
  • Our experts have years of experience in lentiviral cloning and expression.
  • Faster turnaround time, lower price and best service.

Applications

  • Study the biological functions of certain genes/proteins
  • Drug screening and discovery; drug efficacy and toxicity screening; drug combination studies
  • Preclinical safety and toxicity studies

IntegrateRNA has many years of experience in RNA research. With skilled and experienced genomics experts, we are able to provide our clients with professional and efficient shRNA lentiviral construction services to help you design and produce validated shRNA expression constructs, which is confirmed by qRT-PCR to knock down any desired target gene transcripts by at least 75% in the cell lines you are interested. We are committed to providing the most reliable and effective research services to best meet your research goals. If you have any questions, please feel free to contact us.

For research use only. Not intended for any clinical use.
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