Custom mRNA Synthesis Service

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Custom mRNA Synthesis Service

IntegrateRNA provides tailored mRNA synthesis for research use, at milligram to multigram scales, with lengths up to multiple kilobases. We offer numerous post-transcriptional modifications, including DNase and phosphatase treatments, enzymatic capping, and polyadenylation. Additionally, you can select from a variety of purification options, including silica gel purification, liquid chromatography isolation, and high-performance liquid chromatography. Our mRNA services allow researchers to tailor synthesis and introduce modifications to produce transcripts which are optimal for downstream applications.

In vitro synthesized mRNA has been used to study protein-mediated mRNA recognition, mRNA export and translation, and mutational effects in protein-coding genes. It also designed to meet the growing demand for DNA delivery to mammalian cells for in vitro protein expression, genome editing, oocyte microinjection and more. Recently, mRNA-based drugs have been developed for cancer immunotherapy and preventive vaccines. There are two methods to produce customized mRNA:

  • Chemical Synthesis

IntegrateRNA is equipped with various kinds of full-automatic synthesizers, advanced purification equipment, and has rich experience in RNA synthesis, has a perfected set of RNA synthesis and purification technology, can provide mRNA synthesis products with high quality. When your mRNA is shorter than 120 bases, we recommend using chemical synthesis. This is a quick and more economical method.

  • In Vitro Transcription

If you need long fragments of mRNA, we recommend RNA transcript synthesis. The yield of chemically synthesized RNA decreases exponentially with increasing length and is not suitable for synthetic long fragments of RNA. IntegrateRNA has developed a powerful T7 RNA polymerase-based transcription system that synthesizes up to 10 mg of mRNA in vitro in one day. RNA transcript synthesis is an alternative to chemical RNA synthesis and is very useful when long and/or modified capped mRNAs are required.

Production steps

  • Chemical synthesis of dsDNA fragments
  • Cloning of the DNA fragments in a plasmid
  • Quality control by sequencing of plasmid inserts
  • PCR amplification of insert, purification and characterization
  • PCR templates for production of ssRNA by in vitro transcription with T7 RNA polymerase
  • Purification and characterization of the in vitro transcripts

Benefits of our service

  • Scalable and high-quality mRNA manufacturing meeting various requirements.
  • All mRNAs lengths ranging from a few hundred up to several thousand bases.
  • Both research-grade and GMP-grade mRNA can be customized.
  • Different scales: from milligram to multi-gram quantities.
  • Different post-transcriptional modifications, such as DNase and phosphatase treatments, enzymatic capping, and polyadenylation.
  • High quality: ISO 9001 certification, comprehensive quality assurance (HPLC/MS/CGE).
  • Sterility and bioburden controls.

As one of the leading biotechnology companies, IntegrateRNA is dedicated to providing professional mRNA synthesis services. We have comprehensive evaluate existing technologies, optimized processing conditions, and provided a high-quality solution for mRNA synthesis. When ordering mRNA synthesis services, please provide target mRNA sequence or length, base composition or modifications, desired 5' and 3'-UTR sequence, plasmid map, scale, and other valid information. We guarantee faster turnaround times, lower prices.

For research use only. Not intended for any clinical use.
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