Our promise to you:
Guaranteed product quality, expert customer support.
As a global biotechnology company specializing in RNA biology, IntegrateRNA is dedicated to offer high-quality circRNA services for customers. Based on sophisticated equipment, advanced technologies and highly experienced staffs, we provide one stop services for circRNA research, including circRNA prediction, screening, identification, expression analysis, functional analysis and interaction mechanism analysis. Our comprehensive solution will facilitate your research project and explore the potential clinical applications of circRNA.
circRNA is an emerging class of widespread, abundant and evolutionarily conserved noncoding RNA, and represents a research hotspot because of its close association with the development of diseases. Recent studies have suggested that circRNAs play a crucial role in regulating gene expression by acting as a miRNA sponge, RNA binding protein sponge and translational regulator (Figure 1). In addition, due to the features of circRNAs including stability, conservation, high abundance and tissue/developmental stage-specific expression pattern, circRNAs are believed to be potential biomarkers for various diseases.
Figure 1. Function of circRNA
With increasing need to investigate circRNAs with depth and precision, it has become essential to utilize tools and methodologies that investigate accurately circRNA sequence, length, subcellular localization, biological function, disease implication, interacting molecules, and therapeutic potential. IntegrateRNA utilizes many different molecular biology technologies to facility your circRNA research and help you understand complex biological systems (Figure 2).
The whole research of circRNA can be divided into several steps, including prediction, screening, identification, expression analysis, functional analysis and mechanism analysis. IntegrateRNA offers RNA-seq, microarray and bioinformatic analysis services to help you predict and screening target circRNAs under different conditions. qPCR and northern blotting are utilized to further validate the expression of candidate circRNAs. Fluorescence in situ hybridization (FISH) is used in providing localization information about candidate circRNAs. For further function study, we introduce circRNA overexpression and knockdown strategies to manipulate circRNA expression. Besides, we utilized several methods to reveal circRNA-miRNA and circRNA-protein interactions, including bioinformatic prediction, luciferase reporter assay, RNA immunoprecipitation, and RNA pull down. We are confident to provide a one-stop solution to accelerate your circRNA biomarker discovery and preclinical development.
Figure 2. Technique route for circRNA research
Tell us your project needs, we will provide full-length custom circRNA solutions and comprehensive circRNA products. If you want to know more about our services and products, don't hesitate to contact us.