circRNA FISH Service

Services

CBpromise   

Our promise to you:
Guaranteed product quality, expert customer support.

24x7 CUSTOMER SERVICE
CONTACT US TO ORDER

circRNA FISH Service

As a leading biotechnology company specializing in RNA research, IntegrateRNA has launched the latest circRNA FISH solution to help customers determine the presence of circRNA in different cells, as well as detect and localize circRNA, which allow researchers to study circRNA from multiple perspectives, making the experiment more rigorous and convincing.

FISH (fluorescence in situ hybridization) is a new in situ hybridization method formed by directly using fluorophore labeling or indirect biotin labeling instead of isotopic labeling, which enables relative qualitative and localization analysis of target nucleic acid sequences. FISH has been widely used in various fields of biomedical science because of its safety, rapid, sensitivity, long probe retention time and simultaneous display of multiple colors.

The Strategy of circRNA FISH

circRNA FISH Service

Workflow of circRNA FISH Service

  • Probe design (Probe design for circular RNA splice variant detection)
  • Labeling of probes and purification
  • Coverslip preparation
  • Cell fixation — different fixation methods for circRNAs in cytoplasm and nuclear.
  • Permeabilization
  • Post-fixation and preparation for hybridization
  • Hybridization
  • Washing and DAPI staining
  • Imaging and high-precision localizations
  • RNA spot quantification

Advantages

  • Various cell lines (including various cancer cells, primary cells, etc.) and organ samples are processed
  • Electronic picture (common fluorescence microscope or laser confocal microscope)
  • Experimental report (including probe sequence, materials, reagents, instruments, methods, data analysis, experimental results)
  • A cost-effective one-stop solution
  • A team of experienced scientists

Applications

  • circRNA subcellular localization analysis
  • Differential expression analysis of circRNA

IntegrateRNA has many years of experience in RNA research. With skilled and experienced genomics experts, we are able to provide you with professional and efficient circRNA FISH solution to localize circRNA for further study of subsequent functions. We are committed to providing the most reliable and effective research services to help you solve the problems. If you have any questions, please feel free to contact us.

For research use only. Not intended for any clinical use.
Verification code